| Field | Specification |
|---|---|
| Target | |
| Alternative names | Plasma membrane calcium-transporting ATPase 1; Plasma membrane calcium ATPase isoform 1; PMCA1; Plasma membrane calcium pump isoform 1; ATP2B1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PMCA1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22A09 is a rabbit monoclonal antibody that recognizes plasma membrane calcium-transporting ATPase 1 (gene ATP2B1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 134.7 kDa, and the supplier reports an observed band at about 250 kDa.
ATP2B1 (PMCA1) is a plasma membrane Ca2+-ATPase that pumps calcium out of the cell. It is widely expressed and helps keep resting intracellular calcium low, and it is studied in calcium homeostasis and blood pressure regulation.
| Target | Plasma membrane calcium-transporting ATPase 1 (gene ATP2B1; UniProt P20020, human) |
|---|---|
| Clone | 22A09 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 134.7 kDa |
| Observed MW | 250 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HepG2 cells, human U-87MG cells, human U251 cells, human SH-SY5Y cells, rat C6 cells, rat RH35 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PMCA1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places plasma membrane calcium-transporting ATPase 1 in the cell membrane, basolateral cell membrane, synapse and presynaptic cell membrane. Tissue expression noted by UniProt (human): Isoform B: Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
