| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mismatch repair endonuclease PMS2 ; DNA mismatch repair protein PMS2; PMS1 protein homolog 2; PMS2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PMS2 recombinant protein (Position: E656-L767). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mismatch repair endonuclease PMS2 in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 95.8 kDa, and the supplier reports an observed band at about 100 kDa.
PMS2 pairs with MLH1 to form MutLα, a core part of the DNA mismatch repair system that corrects replication errors. After MutS complexes find a mismatch, PMS2 nicks the new DNA strand near the error so that EXO1 can remove and replace it. Loss of PMS2 causes microsatellite instability and is linked to Lynch syndrome.
| Target | Mismatch repair endonuclease PMS2 (gene PMS2; UniProt P54278, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 95.8 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells, human U2OS cells, human MCF-7 cells, human K562 cells, rat C6 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human PMS2 fragment (Glu656–Leu767), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mismatch repair endonuclease PMS2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
