| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PNN/DRSP recombinant protein (Position: A2-L241). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PNN/DRSP Antibody Picoband® is an antibody for PNN detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PNN (neutrophil cytosolic factor 1); UniProt: Q9H307
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 82 kDa, calculated 34140 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-PNN/DRSP Antibody Picoband® catalog # A01590-2.
Biological background
Biological context: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Expression and localization notes: cellular localization: Cytoplasm, cytosol., tissue context: Detected in peripheral blood monocytes and neutrophils (at protein level)..
Common research applications
- Western blotting (WB): Compare PNN levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PNN in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify PNN-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Pinin is a protein that in humans is encoded by the PNN gene. By yeast 2-hybrid analysis of HeLa cells, followed by sequence analysis, it was found that epitope-tagged human PNN interacted with the serine/arginine (SR)-rich proteins SRP75 (SRSF4; 601940), SRM300 (SRRM2; 606032), and SRRP130 (PNISR; 616653). Truncation analysis revealed that the polyserine/RS domain of PNN and flanking sequences participated in binding to these SR proteins. The 4 proteins colocalized in the nucleus of HCE-T cells, and overexpression of any of these proteins affected the distribution of the others between nuclear speckles and nucleoplasm.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm, cytosol.
- Tissue details: Detected in peripheral blood monocytes and neutrophils (at protein level).
- Research category: Cancer,Cancer Metabolism,Energy Metabolism,Energy Transfer Pathways,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
