| Field | Specification |
|---|---|
| Target | |
| Alternative names | POLR1A; DNA-directed RNA polymerase I subunit RPA1; RNA polymerase I subunit A1; EC 2.7.7.6; A190; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; RNA polymerase I 194 kDa subunit; RPA194 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human POLR1A recombinant protein (Position: E607-R1720). Human POLR1A shares 86.3% and 87.3% amino acid (aa) sequence identity with mouse and rat POLR1A, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA-directed RNA polymerase I subunit RPA1 (gene POLR1A) in human samples and is validated for Western blot and ELISA.
POLR1A (RPA194) is the largest subunit of RNA polymerase I, the enzyme that transcribes ribosomal RNA genes in the nucleolus. Pol I makes the 47S pre-rRNA, which is processed into the 18S, 5.8S and 28S rRNAs of the ribosome. Because rRNA synthesis tracks cell growth, POLR1A is studied in ribosome biogenesis and cancer.
| Target | DNA-directed RNA polymerase I subunit RPA1 (gene POLR1A; UniProt O95602, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human POLR1A fragment (Glu607–Arg1720), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 86.3% and 87.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA-directed RNA polymerase I subunit RPA1 in the nucleus and chromosome.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
