| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pro-opiomelanocortin; POMC; Corticotropin-lipotropin |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human POMC ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADEC-16 is a rabbit monoclonal antibody that recognizes pro-opiomelanocortin (gene POMC) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 29.4 kDa, and the supplier reports an observed band at about 29 kDa.
Pro-opiomelanocortin (POMC) is a precursor protein cut into several active peptides, including ACTH, α-, β- and γ-MSH and β-endorphin. These act on melanocortin and opioid receptors to regulate cortisol release, skin pigmentation, appetite and energy balance. POMC is a key marker of pituitary corticotrophs and hypothalamic appetite-regulating neurons.
| Target | Pro-opiomelanocortin (gene POMC; UniProt P01189, human) |
|---|---|
| Clone | ADEC-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.4 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): mouse pituitary.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human POMC ACTH stimulates the adrenal glands to release cortisol.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes pro-opiomelanocortin as a secreted protein. Tissue expression noted by UniProt (human): ACTH and MSH are produced by the pituitary gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
