| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein O-mannosyl-transferase 2; Dolichyl-phosphate-mannose--protein mannosyltransferase 2; POMT2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human POMT2, which shares 61.9% amino acid (aa) sequence identity with both mouse and rat POMT2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-POMT2 Antibody Picoband® is an antibody for POMT2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: POMT2 (protein O-mannosyltransferase 2); UniProt: Q9UKY4
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 84 kDa, calculated 35883 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-POMT2 Antibody Picoband® catalog # A04876-1.
Biological background
Biological context: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.
Expression and localization notes: cellular localization: Endoplasmic reticulum membrane. Multi-pass membrane protein, tissue context: Highly expressed in testis; detected at low levels in most tissues..
Common research applications
- Western blotting (WB): Compare POMT2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of POMT2 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify POMT2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Protein O-mannosyl-transferase 2 is an enzyme that in humans is encoded by the POMT2 gene. This gene is mapped to chromosome 14q24.3. The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT1 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Endoplasmic reticulum membrane. Multi-pass membrane protein
- Tissue details: Highly expressed in testis; detected at low levels in most tissues.
- Research category: Organelles,Subcellular Markers,Tags & Cell Markers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
