| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxisome proliferator-activated receptor gamma;PPAR-gamma;Nuclear receptor subfamily 1 group C member 3;PPARG;NR1C3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PPAR gamma, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects peroxisome proliferator-activated receptor gamma (gene PPARG) in human samples and is validated for Western blot. The predicted molecular weight is 57.6 kDa, and the supplier reports an observed band at about 58 kDa.
PPARγ (PPARG) is a ligand-activated nuclear receptor that works with RXR and is the master regulator of fat cell differentiation. It controls genes for lipid storage and insulin sensitivity and is the target of thiazolidinedione antidiabetic drugs. PPARγ is studied in adipogenesis, metabolism, inflammation and cancer.
| Target | Peroxisome proliferator-activated receptor gamma (gene PPARG; UniProt P37231, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 57.6 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human K562 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human PPAR gamma. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human. UniProt places peroxisome proliferator-activated receptor gamma in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highest expression in adipose tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Hengwei Yu et al. (2024) Comprehensive Analysis of Transcriptome and Metabolome Reveals Regulatory Mechanism of Intramuscular Fat Content in Beef Cattle. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.3c07844
Yang Xi et al. (2018) PIK3R3 regulates PPARα expression to stimulate fatty acid β-oxidation and decrease hepatosteatosis. Experimental and Molecular Medicine. 10.1038/emm.2017.243
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Yeke Wu et al. (2024) A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117738
Gaode Zou et al. (2021) Pioglitazone Ameliorates Renal Ischemia-Reperfusion Injury via Inhibition of NF-κB Activation and Inflammation in Rats. Frontiers in Physiology. 10.3389/fphys.2021.707344
Yang Dai et al. (2022) Asiatic acid protests against myocardial ischemia/reperfusion injury via modulation of glycometabolism in rat cardiomyocyte. Drug Design Development and Therapy. 10.2147/DDDT.S175116
Na Wang et al. (2024) Liraglutide reduces bone marrow adipogenesis by miR-150-5p/ GDF11 axis in diabetic rats. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176793
Yu Zhang et al. (2020) Bovine Stearoyl-CoA Desaturase 1 Promotes Adipogenesis by Activating the PPARγ Receptor. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.0c05147
Chen-Huan Yu et al. (2013) Hypolipidemic and antioxidant activities of polysaccharides from Rosae Laevigatae Fructus in rats. Carbohydrate Polymers. 10.1016/j.carbpol.2013.01.006
