| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxisome proliferator-activated receptor gamma;PPAR-gamma;Nuclear receptor subfamily 1 group C member 3;PPARG;NR1C3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PPAR gamma/PPARG recombinant protein (Position: H58-K329). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects peroxisome proliferator-activated receptor gamma (gene PPARG) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 57.6 kDa, and the supplier reports an observed band at about 65 kDa.
PPARγ (PPARG) is a ligand-activated nuclear receptor that works with RXR and is the master regulator of fat cell differentiation. It controls genes for lipid storage and insulin sensitivity and is the target of thiazolidinedione antidiabetic drugs. PPARγ is studied in adipogenesis, metabolism, inflammation and cancer.
| Target | Peroxisome proliferator-activated receptor gamma (gene PPARG; UniProt P37231, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 57.6 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human MCF-7 cells, human SK-OV-3 cells, human MDA-MB-453 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human PPAR gamma/PPARG fragment (His58–Lys329), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places peroxisome proliferator-activated receptor gamma in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highest expression in adipose tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Yizhou Zhou et al. (2018) Prophylactic effects of triptolide on colon cancer development in azoxymethane/dextran sulfate sodiuminduced mouse model. Tropical Journal of Pharmaceutical Research. 10.4314/tjpr.v16i11.13
