Anti-PPAR gamma Rabbit Monoclonal Antibody

SKU:BHA21008493
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Overview
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Anti-PPARG antibody (Rabbit monoclonal, clone AOFH-16, Rabbit IgG). Validated for Western blot. Reactivity: Human. Recognizes peroxisome proliferator-activated receptor gamma. Observed band in Western blot: about 57 kDa.
Target PPARG
clone number AOFH-16
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M00449 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-1:2000
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target PPARG
Alternative names Peroxisome proliferator-activated receptor gamma;PPAR-gamma;Nuclear receptor subfamily 1 group C member 3;PPARG;NR1C3;
UniProt # P37231
Host Rabbit
Clone AOFH-16
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human PPAR gamma
Molecular weight 57.6 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Cytoplasm. Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. CCRN4L/NOC enhances its nuclear translocation.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00449
Main SKU BHA21008493
Rabbit Monoclonal

Product Overview

Clone AOFH-16 is a rabbit monoclonal antibody that recognizes peroxisome proliferator-activated receptor gamma (gene PPARG) in human samples and is validated for Western blot. The predicted molecular weight is 57.6 kDa, and the supplier reports an observed band at about 57 kDa.

PPARγ (PPARG) is a ligand-activated nuclear receptor that works with RXR and is the master regulator of fat cell differentiation. It controls genes for lipid storage and insulin sensitivity and is the target of thiazolidinedione antidiabetic drugs. PPARγ is studied in adipogenesis, metabolism, inflammation and cancer.

Target Peroxisome proliferator-activated receptor gamma (gene PPARG; UniProt P37231, human)
Clone AOFH-16
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 57.6 kDa
Observed MW 57 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Immunogen

Synthetic peptide based on the sequence of human PPAR gamma.

Reactivity Notes

The supplier lists reactivity with human. UniProt places peroxisome proliferator-activated receptor gamma in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highest expression in adipose tissue.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 57.6 kDa; observed band at about 57 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human PPAR gamma.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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