| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nck-associated protein 1;NAP 1;Membrane-associated protein HEM-2;p125Nap1;NCKAP1;HEM2, KIAA0587, NAP1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PPIL3 recombinant protein (Position: M1-Q161). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PPIL3 Antibody Picoband® is an antibody for PPIL3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PPIL3 (Nck-associated protein 1); UniProt: Q9H2H8
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 18 kDa, calculated 128790 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-PPIL3 Antibody Picoband® catalog # A09246-1.
Biological background
Biological context: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.
Expression and localization notes: cellular localization: Cell membrane ; Single-pass membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Single-pass membrane protein ; Cytoplasmic side . At the interface between the lamellipodial actin meshwork and the membrane. ., tissue context: Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle..
Common research applications
- Western blotting (WB): Compare PPIL3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PPIL3 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify PPIL3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Peptidyl-prolyl cis-trans isomerase-like 3 is an enzyme that in humans is encoded by the PPIL3 gene. This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Alternative splicing results in multiple transcript variants.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane ; Single-pass membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Single-pass membrane protein ; Cytoplasmic side . At the interface between the lamellipodial actin meshwork and the membrane. .
- Tissue details: Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
- Research category: Neurogenesis,Neurology Process,Neuroscience,NFKB Pathway,Nuclear Signaling,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
