| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurabin-1; Neurabin-I; Neural tissue-specific F-actin-binding protein I; Protein phosphatase 1 regulatory subunit 9A; PPP1R9A; KIAA1222 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PPP1R9A recombinant protein (Position: Q204-K1043). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets neurabin-1 (gene PPP1R9A) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 123.3 kDa, and the supplier reports an observed band at about 190 kDa.
Neurabin-1 (PPP1R9A) is an actin-binding scaffold protein enriched in neurons. It binds and crosslinks F-actin and targets protein phosphatase 1 to dendritic spines, where it helps shape spine structure and synaptic plasticity. It is studied in neuronal morphology and synapse function.
| Target | Neurabin-1 (gene PPP1R9A; UniProt Q9ULJ8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 123.3 kDa |
| Observed MW | 190 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1x1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human PC-3 cells, human U251 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human PPP1R9A fragment (Gln204–Lys1043), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places neurabin-1 in the cytoplasm and synapse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
