Anti-PRAME Picoband® Antibody

Overview

Anti-PRAME Picoband® Antibody is an antibody for PRAME detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: PRAME (preferentially expressed antigen in melanoma); UniProt: P78395; NCBI Gene: 23532
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 58 kDa, calculated 104553 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-PRAME Picoband® Antibody catalog # A06628-2.

Biological background

Biological context: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.

Expression and localization notes: cellular localization: Cell membrane. Nucleus., tissue context: Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium..

Common research applications

• Western blotting (WB): Compare PRAME levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of PRAME in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify PRAME-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: Melanoma antigen preferentially expressed in tumors is a protein that in humans is encoded by the PRAME gene. It is mapped to 22q11.22. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Nucleus.
• Tissue details: Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.
• Research category: Cofactors, Vitamins/Minerals,Metabolism,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Pathways and Processes,Signal Transduction,Vitamins/Minerals

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.