| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein regulator of cytokinesis 1 ;PRC1 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PRC1 recombinant protein (Position: M1-E233). Human PRC1 shares 85% amino acid (aa) sequence identity with mouse PRC1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein regulator of cytokinesis 1 (gene PRC1) in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation and Western blot. The predicted molecular weight is 71.6 kDa, and the supplier reports an observed band at about 72 kDa.
PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that crosslinks antiparallel microtubules in the spindle midzone. It organizes the central spindle and recruits motors and kinases such as KIF4A, KIF14 and PLK1, which are needed to complete cytokinesis. PRC1 is studied in cell division and cancer proliferation.
| Target | Protein regulator of cytokinesis 1 (gene PRC1; UniProt O43663, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 71.6 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human U251 cells, human HeLa cells, human 293T cells, human Hacat cells, rat testis tissue, rat liver tissue, mouse testis tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed result (IP): 293T cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human PRC1 fragment (Met1–Glu233), expressed in E. coli. Sequence identity with the mouse ortholog: 85%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein regulator of cytokinesis 1 in the nucleus, cytoplasm, midbody and chromosome. Tissue expression noted by UniProt (human): Overexpressed in bladder cancer cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
