| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prolactin regulatory element-binding protein;Mammalian guanine nucleotide exchange factor mSec12;PREB;SEC12; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human PREB recombinant protein (Position: K42-N236). Human PREB shares 87.7% amino acid (aa) sequence identity with both mouse and rat PREB. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PREB (Vesicular acetylcholine transporter) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PREB Antibody Picoband® catalog # A04946. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human PREB recombinant protein (Position: K42-N236). Human PREB shares 87.7% amino acid (aa) sequence identity with both mouse and rat PREB. (reported region: K42-N236).
- Molecular weight context: reported MW: 45 kDa; calculated MW: 45468 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Vesicular acetylcholine transporter; Prolactin regulatory element-binding protein. Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene. This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand. Functional note: Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER. . Reported localization: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus . Concentrates at endoplasmic reticulum exit sites. . Expression/tissue context: Ubiquitous. .
Research relevance and current trends
- Epigenetics and Nuclear Signaling: Researchers commonly examine how PREB (Vesicular acetylcholine transporter) relates to this theme using model systems and orthogonal readouts.
- Transcription: Researchers commonly examine how PREB (Vesicular acetylcholine transporter) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PREB (Vesicular acetylcholine transporter) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
