| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prolactin regulatory element-binding protein;Mammalian guanine nucleotide exchange factor mSec12;PREB;SEC12; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PREB recombinant protein (Position: K42-N236). Human PREB shares 87.7% amino acid (aa) sequence identity with both mouse and rat PREB. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets guanine nucleotide-exchange factor SEC12 (gene PREB) in human samples and is validated for Western blot. The predicted molecular weight is 45.5 kDa, and the supplier reports an observed band at about 45 kDa.
SEC12 (PREB) is an endoplasmic reticulum membrane protein that acts as the guanine nucleotide exchange factor for the small GTPases SAR1A and SAR1B. By loading SAR1 with GTP it starts assembly of the COPII coat that buds vesicles from the ER toward the Golgi; it was first described as a prolactin promoter-binding factor in the pituitary.
| Target | Guanine nucleotide-exchange factor SEC12 (gene PREB; UniProt Q9HCU5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 45.5 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human placenta tissue, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human PREB fragment (Lys42–Asn236), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 87.7%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places guanine nucleotide-exchange factor SEC12 in the endoplasmic reticulum membrane and nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
