| Field | Specification |
|---|---|
| Target | |
| Alternative names | Monocyte differentiation antigen CD14 ;Myeloid cell-specific leucine-rich glycoprotein;CD14;Monocyte differentiation antigen CD14, urinary form;Monocyte differentiation antigen CD14, membrane-bound form;CD14; |
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| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Presenilin 1 Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein) . Requires the other members of the gamma-secretase complex to have a protease activity. |
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| Form | Liquid |
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Overview
This product is an anti-PSEN1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AECD-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, Flow (as provided in the source record). Boster Bio Anti-Presenilin 1 Monoclonal Antibody catalog # M00138-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PSEN1 (Monocyte differentiation antigen CD14).
- Antibody format: Monoclonal; clone AECD-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PSEN1 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL (-)) and mediates the cytokine release induced by LDL (-) (PubMed:23880187). . Reported cellular localization context: Cell membrane ; Lipid- anchor, GPI-anchor . Secreted . Membrane raft . Golgi apparatus . Secreted forms may arise by cleavage of the GPI anchor. . Tissue expression notes (as provided): Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. .
Research relevance and current trends
- Research context keywords from the source record include: Alzheimer's Disease,Cancer,Cell Death,Metabolism,Metabolism Processes,Neurodegenerative Disease,Neurology Process,Neuroscience,Pathways and Processes,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate PSEN1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PSEN1 expression by Western blot in cell or tissue lysates, Quantify PSEN1-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 25 kDa; calculated MW: 40076 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 25 kDa
- Cellular localization (provided): Cell membrane ; Lipid- anchor, GPI-anchor . Secreted . Membrane raft . Golgi apparatus . Secreted forms may arise by cleavage of the GPI anchor. .
- Tissue details (provided): Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
