| Field | Specification |
|---|---|
| Target | |
| Alternative names | Presenilin-1; PS-1; Protein S182; PSEN1; AD3; PS1; PSNL1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Presenilin 1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AECD-16 is a rabbit monoclonal antibody that recognizes presenilin-1 (gene PSEN1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 52.7 kDa, and the supplier reports an observed band at about 28 kDa.
Presenilin-1 (PSEN1) is the catalytic subunit of γ-secretase, an intramembrane aspartyl protease complex with nicastrin, APH-1 and PEN-2. γ-Secretase cleaves type I membrane proteins such as Notch and APP, releasing Notch signaling fragments and amyloid-β peptides, and PSEN1 mutations are the most common cause of early-onset familial Alzheimer's disease.
| Target | Presenilin-1 (gene PSEN1; UniProt P49768, human) |
|---|---|
| Clone | AECD-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.7 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:10 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human presenilin 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places presenilin-1 in the endoplasmic reticulum, endoplasmic reticulum membrane, Golgi apparatus membrane and cytoplasmic granule. Tissue expression noted by UniProt (human): Detected in azurophile granules in neutrophils and in platelet cytoplasmic granules (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
