| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein arginine N-methyltransferase 5;2.1.1.320 ;72 kDa ICln-binding protein;Histone-arginine N-methyltransferase PRMT5;Jak-binding protein 1;Shk1 kinase-binding protein 1 homolog;SKB1 homolog;SKB1Hs;Protein arginine N-methyltransferase 5, N-terminally processed;PRMT5;HRMT1L5, IBP72, JBP1, SKB1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PRMT5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DCE-16 is a rabbit monoclonal antibody that recognizes protein arginine N-methyltransferase 5 (gene PRMT5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 72.7 kDa, and the supplier reports an observed band at about 71 kDa.
PRMT5 is the principal type II protein arginine methyltransferase, forming symmetric dimethylarginine together with its partner WDR77 (MEP50). It methylates Sm proteins needed for spliceosomal snRNP assembly as well as histones and transcription factors, and it has become an important target in cancer research, particularly in MTAP-deleted tumors.
| Target | Protein arginine N-methyltransferase 5 (gene PRMT5; UniProt O14744, human) |
|---|---|
| Clone | DCE-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 72.7 kDa |
| Observed MW | 71 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human MCF-7 cells, human K562 cells, rat spleen tissue, rat brain tissue, mouse spleen tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PRMT5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein arginine N-methyltransferase 5 in the cytoplasm, nucleus, chromosome and Golgi apparatus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
