| Field | Specification |
|---|---|
| Target | |
| Alternative names | Progesterone receptor;PR;Nuclear receptor subfamily 3 group C member 3;PGR;NR3C3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Progesterone Receptor, identical to the related rat and mouse sesquences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets progesterone receptor (gene PGR) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 99.0 kDa, and the supplier reports an observed band at about 36 kDa.
The progesterone receptor (PGR) is a ligand-activated nuclear receptor that controls gene expression in response to progesterone. Its two main isoforms, PR-A and PR-B, have partly different activities, and signaling through them regulates the uterus, ovary and mammary gland. PR status is routinely assessed in breast cancer research.
| Target | Progesterone receptor (gene PGR; UniProt P06401, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 99.0 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the middle region of human progesterone Receptor.
Reactivity Notes
The supplier lists reactivity with human. UniProt places progesterone receptor in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ruddenklau Amy et al. (2023) Validation of a new Custom Polyclonal Progesterone Receptor Antibody for Immunohistochemistry in the Female Mouse Brain. Journal of the Endocrine Society. 10.1210/jendso/bvad113
