| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prokineticin-1; Endocrine-gland-derived vascular endothelial growth factor; EG-VEGF; Mambakine; PROK1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PROK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26P23 is a rabbit monoclonal antibody that recognizes prokineticin-1 (gene PROK1) in human and mouse samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 11.7 kDa, and the supplier reports an observed band at about 14 kDa.
Prokineticin 1 (PROK1, also called EG-VEGF) is a small secreted protein that signals through the G protein-coupled receptors PROKR1 and PROKR2. It stimulates contraction of gastrointestinal smooth muscle and promotes angiogenesis in endocrine glands such as the ovary, testis and placenta, and it is studied in reproduction and pregnancy.
| Target | Prokineticin-1 (gene PROK1; UniProt P58294, human) |
|---|---|
| Clone | 26P23 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.7 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PROK1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes prokineticin-1 as a secreted protein. Tissue expression noted by UniProt (human): Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
