| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prostaglandin E2 receptor EP4 subtype;PGE receptor EP4 subtype;PGE2 receptor EP4 subtype;Prostanoid EP4 receptor;PTGER4;PTGER2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PTGER4, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PTGER4 (Prostaglandin E2 receptor EP4 subtype) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Prostaglandin E Receptor EP4/PTGER4 Antibody Picoband® catalog # A02153-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human PTGER4, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 53 kDa; calculated MW: 53119 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Prostaglandin E2 receptor EP4 subtype; Prostaglandin E2 receptor EP4 subtype. Prostaglandin E2 receptor 4 (EP4) is a prostaglandin receptor encoded by the PTGER4 gene in humans. The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. Functional note: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G (s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Reported localization: Cell membrane; Multi-pass membrane protein. Expression/tissue context: High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
Research relevance and current trends
- G Protein Signaling: Researchers commonly examine how PTGER4 (Prostaglandin E2 receptor EP4 subtype) relates to this theme using model systems and orthogonal readouts.
- Lipid Signaling: Researchers commonly examine how PTGER4 (Prostaglandin E2 receptor EP4 subtype) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how PTGER4 (Prostaglandin E2 receptor EP4 subtype) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PTGER4 (Prostaglandin E2 receptor EP4 subtype) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
