| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | cAMP-dependent protein kinase type I-alpha regulatory subunit;Tissue-specific extinguisher 1;TSE1;cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed;PRKAR1A;PKR1, PRKAR1, TSE1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PRKAR1A (cAMP-dependent protein kinase type I-alpha regulatory subunit) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband® catalog # A00699-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively. (reported region: E2-E81).
- Molecular weight context: reported MW: 48 kDa; calculated MW: 42982 MW
- Reactivity: Human,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
cAMP-dependent protein kinase type I-alpha regulatory subunit; cAMP-dependent protein kinase type I-alpha regulatory subunit. cAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. This protein encoded by this gene was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed. Functional note: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. . Reported localization: Cell membrane . Expression/tissue context: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Research relevance and current trends
- Cancer: Researchers commonly examine how PRKAR1A (cAMP-dependent protein kinase type I-alpha regulatory subunit) relates to this theme using model systems and orthogonal readouts.
- Cell Cycle: Researchers commonly examine how PRKAR1A (cAMP-dependent protein kinase type I-alpha regulatory subunit) relates to this theme using model systems and orthogonal readouts.
- DNA/RNA: Researchers commonly examine how PRKAR1A (cAMP-dependent protein kinase type I-alpha regulatory subunit) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PRKAR1A (cAMP-dependent protein kinase type I-alpha regulatory subunit) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of PRKAR1A (cAMP-dependent protein kinase type I-alpha regulatory subunit) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
