| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prothrombin; Coagulation factor II; Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain; F2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Prothrombin recombinant protein (Position: Y67-R124). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets prothrombin (gene F2) in human samples and is validated for ELISA, flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 70.0 kDa, and the supplier reports an observed band at about 85 kDa.
Prothrombin (F2) is the vitamin K-dependent precursor of thrombin, the central protease of blood coagulation. Thrombin converts fibrinogen to fibrin, activates factors V, VIII, XI and XIII and platelets through PAR receptors, and with thrombomodulin activates the anticoagulant protein C. The prothrombin G20210A variant is a well-known risk factor for venous thrombosis.
| Target | Prothrombin (gene F2; UniProt P00734, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 70.0 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): human PBMC cells.
Immunogen
Recombinant human Prothrombin fragment (Tyr67–Arg124), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes prothrombin as a secreted protein. Tissue expression noted by UniProt (human): Expressed by the liver and secreted in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Changhong et al. (2021) Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma. Frontiers in Oncology. 10.3389/fonc.2021.621806
Zhenghang Li et al. (2024) An Apoptosis-Related Specific Risk Model for Breast Cancer: From Genomic Analysis to Precision Medicine. Frontiers in Bioscience-Landmark. 10.31083/j.fbl2907239
