| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prosaposin; Proactivator polypeptide; PSAP; GLBA; SAP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PSAP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24P28 is a rabbit monoclonal antibody that recognizes prosaposin (gene PSAP) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 58.1 kDa, and the supplier reports an observed band at about 78 kDa.
Prosaposin (PSAP) is the precursor of four small lysosomal proteins, saposins A to D, which help acid hydrolases break down sphingolipids by lifting lipid substrates out of membranes. Uncleaved prosaposin is also secreted and has neurotrophic activity, and PSAP deficiency causes lysosomal lipid storage disorders.
| Target | Prosaposin (gene PSAP; UniProt P07602, human) |
|---|---|
| Clone | 24P28 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.1 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PSAP.
Reactivity Notes
The supplier lists reactivity with human. UniProt places prosaposin in the lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
