| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prostate stem cell antigen;PSCA;UNQ206/PRO232; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PSCA (Prostate stem cell antigen) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PSCA Antibody Picoband® catalog # PB9351. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA. (reported region: L21-S95).
- Molecular weight context: reported MW: 25 kDa; calculated MW: 12912 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Prostate stem cell antigen; Prostate stem cell antigen. PSCA is also known as PRO232. This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants. Functional note: May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. . Reported localization: Cell membrane; Lipid-anchor, GPI-anchor. Expression/tissue context: Highly expressed in prostate (basal, secretory and neuroendocrine epithelium cells). Also found in bladder (transitional epithelium), placenta (trophoblasts), stomach (neuroendocrine cells), colon (neuroendocrine cells) and kidney (collecting ducts). Overexpressed in prostate cancers and expression is correlated with tumor stage, grade and androgen- independence. Highly expressed in prostate cancer bone metastases. Expressed in gastric epithelial cells, mainly in the isthmus (at protein level). Not detected in normal intestinal epithelium (at protein level). .
Research relevance and current trends
- Cell Type Markers: Researchers commonly examine how PSCA (Prostate stem cell antigen) relates to this theme using model systems and orthogonal readouts.
- Stem Cells: Researchers commonly examine how PSCA (Prostate stem cell antigen) relates to this theme using model systems and orthogonal readouts.
- Tags & Cell Markers: Researchers commonly examine how PSCA (Prostate stem cell antigen) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PSCA (Prostate stem cell antigen) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Family / similarity context: Contains 1 UPAR/Ly6 domain.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
