| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prostate stem cell antigen;PSCA;UNQ206/PRO232; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets prostate stem cell antigen (gene PSCA) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 12.0 kDa, and the supplier reports an observed band at about 25 kDa.
Prostate stem cell antigen (PSCA) is a small GPI-anchored cell surface protein of the Ly-6/uPAR family. It is expressed in normal prostate, bladder and stomach epithelium and is overexpressed in several carcinomas, and, like other Ly-6 proteins, it can modulate nicotinic acetylcholine receptors.
| Target | Prostate stem cell antigen (gene PSCA; UniProt O43653, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 12.0 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): rat stomach tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human PSCA fragment (Leu21–Ser95), expressed in E. coli. Sequence identity with the mouse ortholog: 66%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places prostate stem cell antigen in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in prostate (basal, secretory and neuroendocrine epithelium cells). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.