| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PH and SEC7 domain-containing protein 1; Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6; Exchange factor for ARF6; Exchange factor for ARF6 A; Pleckstrin homology and SEC7 domain-containing protein 1; PSD; EFA6, EFA6A |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human PSD recombinant protein (Position: T536-K803). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PSD (Keratin, type II cytoskeletal 8) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PSD Antibody Picoband® catalog # A01427. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human PSD recombinant protein (Position: T536-K803). (reported region: T536-K803).
- Molecular weight context: reported MW: 130 kDa; calculated MW: 51548 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Keratin, type II cytoskeletal 8; pleckstrin and Sec7 domain containing. PSD, which called EFA6A, is 63% identical to EFA6B (PSD4) and contains a Sec7 domain adjacent to a PH domain, followed by a C-terminal coiled-coil motif. This gene is mapped to chromosome 10q24. And it encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants. Functional note: Guanine nucleotide exchange factor for ARF6 (PubMed:23603394). Induces cytoskeletal remodeling (By similarity). Reported localization: Cell membrane. Expression/tissue context: Isoform 2 is expressed in the brain.
Research relevance and current trends
- Cytokines: Researchers commonly examine how PSD (Keratin, type II cytoskeletal 8) relates to this theme using model systems and orthogonal readouts.
- Hematopoietic Progenitors: Researchers commonly examine how PSD (Keratin, type II cytoskeletal 8) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how PSD (Keratin, type II cytoskeletal 8) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PSD (Keratin, type II cytoskeletal 8) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
