| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutamate carboxypeptidase 2;3.4.17.21;Cell growth-inhibiting gene 27 protein;Folate hydrolase 1;Folylpoly-gamma-glutamate carboxypeptidase;FGCP;Glutamate carboxypeptidase II;GCPII;Membrane glutamate carboxypeptidase;mGCP;N-acetylated-alpha-linked acidic dipeptidase I;NAALADase I;Prostate-specific membrane antigen;PSM;PSMA;Pteroylpoly-gamma-glutamate carboxypeptidase;FOLH1;FOLH, NAALAD1, PSM, PSMA;GIG27; |
| Gene ID | |
| UniProt # | |
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| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PSMA |
| Molecular weight | |
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| Concentration | |
| Form | Liquid |
| Storage | |
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Product Overview
Clone BBD-6 is a rabbit monoclonal antibody that recognizes glutamate carboxypeptidase 2 (gene FOLH1) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 84.3 kDa, and the supplier reports an observed band at about 110 kDa.
Glutamate carboxypeptidase II (FOLH1), better known as prostate-specific membrane antigen (PSMA), is a membrane-bound zinc peptidase. In the intestine it releases folate from dietary folate polyglutamates, and in the brain it cleaves the neuropeptide NAAG. PSMA is strongly expressed in prostate cancer and is widely used as an imaging and targeting marker.
| Target | Glutamate carboxypeptidase 2 (gene FOLH1; UniProt Q04609, human) |
|---|---|
| Clone | BBD-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 84.3 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): LnCaP cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human prostate carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PSMA.
Reactivity Notes
The supplier lists reactivity with human. UniProt places glutamate carboxypeptidase 2 in the cell membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Highly expressed in prostate epithelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Jiao et al. (2023) Landscape of infiltrating immune cells and related genes in diabetic kidney disease. Clinical and Experimental Nephrology. 10.1007/s10157-023-02422-1
