| Field | Specification |
|---|---|
| Target | |
| Alternative names | Polypyrimidine tract-binding protein 2;Neural polypyrimidine tract-binding protein;Neurally-enriched homolog of PTB;PTB-like protein;PTBP2;NPTB, PTB, PTBLP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PTBP2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABF-16 is a rabbit monoclonal antibody that recognizes polypyrimidine tract-binding protein 2 (gene PTBP2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 57.5 kDa, and the supplier reports an observed band at about 57 kDa.
PTBP2 (also called nPTB) is a neuron- and testis-enriched paralog of PTBP1 that binds pyrimidine-rich intronic sequences to repress alternative exons. The switch from PTBP1 to PTBP2 during neuronal differentiation remodels splicing programs needed for neuronal maturation, so it is studied in neurodevelopment.
| Target | Polypyrimidine tract-binding protein 2 (gene PTBP2; UniProt Q9UKA9, human) |
|---|---|
| Clone | ABF-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.5 kDa |
| Observed MW | 57 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PTBP2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places polypyrimidine tract-binding protein 2 in the nucleus. Tissue expression noted by UniProt (human): Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
