| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human PTCRA recombinant protein (Position: T24-T143). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PTCRA (Protein NDRG3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PTCRA Antibody Picoband® catalog # A10240-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human PTCRA recombinant protein (Position: T24-T143). (reported region: T24-T143).
- Molecular weight context: reported MW: 29 kDa; calculated MW: 41409 MW
- Reactivity: Human
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein NDRG3; pre T cell antigen receptor alpha. This PTCRA gene is mapped to 6p21.1. The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene. Functional note: The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. . Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Research relevance and current trends
- Hematopoietic Progenitors: Researchers commonly examine how PTCRA (Protein NDRG3) relates to this theme using model systems and orthogonal readouts.
- Lymphoid: Researchers commonly examine how PTCRA (Protein NDRG3) relates to this theme using model systems and orthogonal readouts.
- Stem Cells: Researchers commonly examine how PTCRA (Protein NDRG3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PTCRA (Protein NDRG3) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
