| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PTCRA recombinant protein (Position: T24-T143). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets pre T-cell antigen receptor alpha (gene PTCRA) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 29.3 kDa, and the supplier reports an observed band at about 29 kDa.
Pre-Tα (PTCRA) is the invariant chain that pairs with a newly rearranged TCRβ chain and the CD3 complex to form the pre-T-cell receptor. Signaling through this receptor drives β-selection in the thymus, allowing developing thymocytes to proliferate and progress toward the αβ T-cell lineage.
| Target | Pre T-cell antigen receptor alpha (gene PTCRA; UniProt Q6ISU1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 29.3 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A375 cells, human HL-60 cells, human CCRM-CEM cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human PTCRA fragment (Thr24–Thr143), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places pre T-cell antigen receptor alpha in the membrane and cell membrane. Tissue expression noted by UniProt (human): Expressed in immature but not mature T-cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
