| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prostate tumor-overexpressed gene 1 protein;PTOV-1;Activator interaction domain-containing protein 2;PTOV1;ACID2;PP642, UNQ6127/PRO20092; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PTOV1, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PTOV1 (Prostate tumor-overexpressed gene 1 protein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PTOV1 Antibody catalog # RP1087. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human PTOV1, different from the related mouse and rat sequences by one amino acid.
- Molecular weight context: reported MW: 47 kDa; calculated MW: 46869 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Prostate tumor-overexpressed gene 1 protein; Prostate tumor-overexpressed gene 1 protein. Prostate tumor overexpressed gene 1 protein is a protein that in humans is encoded by the PTOV1 gene. It is mapped to 19q13.33. This gene encodes a protein that was found to be overexpressed in prostate adenocarcinomas. The encoded protein was found to interact with the lipid raft protein flotillin-1 and shuttle it from the cytoplasm to the nucleus in a cell cycle dependent manner. Alternative splicing of this gene results in multiple transcript variants. Functional note: May activate transcription. Required for nuclear translocation of FLOT1. Promotes cell proliferation. . Reported localization: Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocates from the cytoplasm to the nucleus at the onset of S-phase. Also localizes to lipid rafts. Expression/tissue context: Expressed in brain, heart, kidney, liver, placenta, skeletal muscle and small intestine. .
Research relevance and current trends
- Cancer: Researchers commonly examine how PTOV1 (Prostate tumor-overexpressed gene 1 protein) relates to this theme using model systems and orthogonal readouts.
- Cell Cycle: Researchers commonly examine how PTOV1 (Prostate tumor-overexpressed gene 1 protein) relates to this theme using model systems and orthogonal readouts.
- Cell Division: Researchers commonly examine how PTOV1 (Prostate tumor-overexpressed gene 1 protein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PTOV1 (Prostate tumor-overexpressed gene 1 protein) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the Mediator complex subunit 25 family. PTOV1 subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
