Anti-PU.1/Spi1 Antibody

Overview

This antibody is intended for detection of SPI1 (Transcription factor PU.1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-PU.1/Spi1 Antibody catalog # RP1097. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196). Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively. (reported region: E18-K196).
• Molecular weight context: reported MW: 150 kDa; calculated MW: 31083 MW
• Reactivity: Human,Mouse,Rat
• Applications: IHC

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Transcription factor PU.1; Transcription factor PU.1. Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. Functional note: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). . Reported localization: Nucleus .

Research relevance and current trends

• Adaptive Immunity: Researchers commonly examine how SPI1 (Transcription factor PU.1) relates to this theme using model systems and orthogonal readouts.
• B Cells: Researchers commonly examine how SPI1 (Transcription factor PU.1) relates to this theme using model systems and orthogonal readouts.
• DNA/RNA: Researchers commonly examine how SPI1 (Transcription factor PU.1) relates to this theme using model systems and orthogonal readouts.

Common research applications

• IHC/IHC-F: assess spatial distribution of SPI1 (Transcription factor PU.1) across tissue regions and cell types using matched controls.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.