| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2-binding component 3, isoforms 1/2; JFY-1; p53 up-regulated modulator of apoptosis; BBC3; PUMA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PUMA/BBC3, which shares 95.7% amino acid (aa) sequence identity with mouse and rat BBC3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes bcl-2-binding component 3, isoforms 1/2 (gene BBC3) in human samples and is validated for Western blot. The predicted molecular weight is 20.5 kDa, and the supplier reports an observed band at about 18 kDa.
PUMA (BBC3) is a BH3-only pro-apoptotic Bcl-2 family protein. It is strongly induced by p53 after DNA damage and also by p53-independent stresses, and it binds and neutralizes anti-apoptotic proteins such as Bcl-2 and Bcl-xL, freeing BAX and BAK to trigger mitochondrial apoptosis.
| Target | Bcl-2-binding component 3, isoforms 1/2 (gene BBC3; UniProt Q9BXH1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 20.5 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human) |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human K562 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human PUMA/BBC3. Sequence identity with the mouse and rat orthologs: 95.7%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places bcl-2-binding component 3, isoforms 1/2 in the mitochondrion. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
