| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ras-related protein Rab-11B;GTP-binding protein YPT3; RAB11B;YPT3 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RAB11B, which shares 97.4% and 100% amino acid (aa) sequence identity with mouse and rat RAB11B, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-RAB11B Antibody Picoband® (monoclonal, 6C5) is an antibody for RAB11B detection raised in Mouse (Monoclonal, clone Clone: 6C5, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: RAB11B (RAB11B,member RAS oncogene family); UniProt: Q15907
- Antibody format: Mouse, Monoclonal, clone Clone: 6C5, Mouse IgG2b
- Molecular weight: 24 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-RAB11B Antibody Picoband® (monoclonal, 6C5) catalog # M04526.
Biological background
Biological context: The small GTPases Rab are key regulators of intracellular membrane trafficking,from the formation of transport vesicles to their fusion with membranes.Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors ly responsible for vesicle formation,movement,tethering and fusion.That Rab plays a role in endocytic recycling,regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR,epithelial sodium channel/ENaC,potassium voltage- gated channel,and voltage-dependent L-type calcium channel.May also regulate constitutive and regulated secretion,like insulin granule exocytosis.Required for melanosome transport and release from melanocytes.Also regulates V-ATPase intracellular transport in response to extracellular acidosis.
Expression and localization notes: cellular localization: Recycling endosome membrane;Lipid-anchor;Cytoplasmic side.Cytoplasmic vesicle,secretory vesicle,synaptic vesicle membrane;Lipid-anchor;Cytoplasmic side.Cytoplasmic vesicle,phagosome membrane;Lipid-anchor;Cytoplasmic side.Recruited to phagosomes containing S.aureus., tissue context: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells..
Common research applications
- Western blotting (WB): Compare RAB11B levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of RAB11B in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify RAB11B-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Ras-related protein Rab-11B is a protein that in humans is encoded by the RAB11B gene. It is mapped to 19p13.2. The Ras superfamily of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Recycling endosome membrane;Lipid-anchor;Cytoplasmic side.Cytoplasmic vesicle,secretory vesicle,synaptic vesicle membrane;Lipid-anchor;Cytoplasmic side.Cytoplasmic vesicle,phagosome membrane;Lipid-anchor;Cytoplasmic side.Recruited to phagosomes containing S.aureus.
- Tissue details: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
- Research category: Cell Adhesion Proteins,Epigenetics and Nuclear Signaling,Integrins,Mediator Complex,Neuroscience,Receptors,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
