| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ras-related protein Rab-3A;RAB3A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3A, different from the related mouse and rat sequences by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of RAB3A (Ras-related protein Rab-3A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Rab3A Antibody Picoband® catalog # PB9322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3A, different from the related mouse and rat sequences by two amino acids.
- Molecular weight context: reported MW: 25 kDa; calculated MW: 24984 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Ras-related protein Rab-3A; Ras-related protein Rab-3A. Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. This gene is mapped to 19p13.11. Synapsin I stimulated the RAB3A cycle by increasing GTP binding, GTPase activity, and RAB3A recruitment to the synaptic vesicle membrane. Conversely, RAB3A inhibited synapsin I binding to actin and synapsin I-induced synaptic vesicle clustering. It has been found that RAB3A was necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, RAB3A has been found to regulate a late step in synaptic vesicle fusion. Functional note: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. Reported localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Expression/tissue context: Specifically expressed in brain.
Research relevance and current trends
- G Protein Signaling: Researchers commonly examine how RAB3A (Ras-related protein Rab-3A) relates to this theme using model systems and orthogonal readouts.
- Ras Family: Researchers commonly examine how RAB3A (Ras-related protein Rab-3A) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how RAB3A (Ras-related protein Rab-3A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative RAB3A (Ras-related protein Rab-3A) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
