| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras-related protein Rab-5A;RAB5A;RAB5; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Rab5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EOE-18 is a rabbit monoclonal antibody that recognizes ras-related protein Rab-5A (gene RAB5A) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 23.7 kDa, and the supplier reports an observed band at about 24 kDa.
Rab5A (RAB5A) is the classic early endosome Rab GTPase. It drives fusion of endocytic vesicles with early endosomes by recruiting effectors such as EEA1 and Rabaptin-5 and is required for uptake of EGF and transferrin, making it a standard early endosome marker.
| Target | Ras-related protein Rab-5A (gene RAB5A; UniProt P20339, human) |
|---|---|
| Clone | EOE-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.7 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells, human K562 cells, human U251 cells, rat brain tissue, mouse brain tissueIHC: human colon tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human rab5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ras-related protein Rab-5A in the cell membrane, early endosome membrane and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
