| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras-related protein Rab-9A;RAB9A;RAB9; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ras-related protein Rab-9A (gene RAB9A) in human, monkey and mouse samples and is validated for Western blot, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 22.8 kDa, and the supplier reports an observed band at about 23 kDa.
Rab9A (RAB9A) is a Rab GTPase on late endosomes that controls retrograde transport of cargo, such as mannose 6-phosphate receptors, back to the trans-Golgi network. It uses dynein-linked effectors for this movement and also contributes to melanosome biogenesis.
| Target | Ras-related protein Rab-9A (gene RAB9A; UniProt P51151, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.8 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Monkey, Mouse) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| Immunoprecipitation | Not specified by the supplier |
Samples with a confirmed band (WB): human K562 cells, human HepG2 cells, human 293T cells, monkey COS-7 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Synthetic peptide from the C-terminal region of human rab9. It differs from the mouse sequence at three residues and from the rat sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human, monkey and mouse. UniProt places ras-related protein Rab-9A in the cell membrane, endoplasmic reticulum membrane and Golgi apparatus membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
