| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysine-specific demethylase RAD23A; UV excision repair protein RAD23 homolog A; HR23A; hHR23A; RAD23A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RAD23A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23R81 is a rabbit monoclonal antibody that recognizes lysine-specific demethylase RAD23A in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 39.6 kDa, and the supplier reports an observed band at about 55 kDa.
RAD23A (HR23A) is a ubiquitin-binding shuttle protein with a ubiquitin-like domain. It helps deliver ubiquitinated proteins to the proteasome and, together with XPC, participates in recognizing DNA damage in nucleotide excision repair.
| Target | Lysine-specific demethylase RAD23A (gene RAD23A; UniProt P54725, human) |
|---|---|
| Clone | 23R81 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.6 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RAD23A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places lysine-specific demethylase RAD23A in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
