| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA repair protein RAD50; hRAD50; RAD50 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Rad50. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACCG-18 is a rabbit monoclonal antibody that recognizes DNA repair protein RAD50 in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 153.9 kDa, and the supplier reports an observed band at about 154 kDa.
RAD50 is an ATPase of the SMC family that forms the MRN complex with MRE11 and NBS1. MRN detects DNA double-strand breaks, activates ATM, begins end resection for homologous recombination and helps maintain telomeres, so RAD50 is central to studies of DNA damage signaling and repair.
| Target | DNA repair protein RAD50 (gene RAD50; UniProt Q92878, human) |
|---|---|
| Clone | ACCG-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 153.9 kDa |
| Observed MW | 154 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human rad50.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA repair protein RAD50 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed at very low level in most tissues, except in testis where it is expressed at higher level. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
