| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ubiquitin-like modifier-activating enzyme ATG7;ATG12-activating enzyme E1 ATG7;Autophagy-related protein 7;APG7-like;hAGP7;Ubiquitin-activating enzyme E1-like protein;ATG7;APG7L; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Rad50 Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-RAD50 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ACCG-18; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF (as provided in the source record). Boster Bio Anti-Rad50 Monoclonal Antibody catalog # M00347. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: RAD50 (Ubiquitin-like modifier-activating enzyme ATG7).
- Antibody format: Monoclonal; clone ACCG-18; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
RAD50 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. . Reported cellular localization context: Cytoplasm . Preautophagosomal structure . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. . Tissue expression notes (as provided): Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .
Research relevance and current trends
- Research context keywords from the source record include: Chromosome Structure,DNA/RNA,DNA Damage & Repair,Epigenetics and Nuclear Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate RAD50 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect RAD50 expression by Western blot in cell or tissue lysates, Localize RAD50 by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 35 kDa; calculated MW: 77960 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 35 kDa
- Cellular localization (provided): Cytoplasm . Preautophagosomal structure . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .
- Tissue details (provided): Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
