| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2; RAD51C; RAD51L2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RAD51C recombinant protein (Position: Q11-K342). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes DNA repair protein RAD51 homolog 3 (gene RAD51C) in human and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 42.2 kDa, and the supplier reports an observed band at about 42 kDa.
RAD51C is a RAD51 paralog found in both the BCDX2 and CX3 complexes, which act at early and late stages of homologous recombination. It is needed for efficient RAD51 foci formation and DNA repair, and germline RAD51C mutations are linked to hereditary breast and ovarian cancer.
| Target | DNA repair protein RAD51 homolog 3 (gene RAD51C; UniProt O43502, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 42.2 kDa |
| Observed MW | 42 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human A549 cells, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human adrenocortical adenoma tissue, human breast cancer tissue, human larynx squamous cells carcinoma tissue, human liver cancer tissue, human testicular seminoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): RT4 cells.
Immunogen
Recombinant human RAD51C fragment (Gln11–Lys342), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places DNA repair protein RAD51 homolog 3 in the nucleus, cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
