| Field | Specification |
|---|---|
| Purity |
Product Overview
Research Use OnlyThis gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2.
Validated Applications
| Western Blot (WB) | 1:500~1:1000 IHC: 1:50~1:100 |
|---|---|
| Immunohistochemistry (IHC) | 1:50~1:100 |
Key Specifications
| Clonality | Polyclonal |
|---|---|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Catalog No. | Y021006 |
| Alternative Names | Raf-1, c-Raf, CRAF |
Immunogen / Antigen
This antiserum was produced against synthesized non-phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-SP-T-P).
Reactivity Notes
Raf-1 (Ab-259) antibody detects endogenous levels of total Raf-1 protein.
Additional Information
Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100
Storage & Handling
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunohistochemistry. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:500~1:1000 IHC: 1:50~1:100
This antibody has been confirmed reactive against: Human,Mouse,Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
This antiserum was produced against synthesized non-phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-SP-T-P).
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
