| Field | Specification |
|---|---|
| Target | |
| Alternative names | Advanced glycosylation end product-specific receptor;Receptor for advanced glycosylation end products;AGER;RAGE; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human RAGE, different from the related mouse and rat sequences by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets advanced glycation end product-specific receptor (gene AGER) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 42.8 kDa, and the supplier reports an observed band at about 45–55 kDa.
RAGE (AGER, receptor for advanced glycation end products) is a cell surface pattern recognition receptor of the immunoglobulin superfamily. It binds diverse stress-related ligands, including advanced glycation end products, S100 proteins, HMGB1 and amyloid-β oligomers, and triggers inflammatory signaling, so it is studied in diabetes complications, vascular disease, neurodegeneration and chronic inflammation.
| Target | Advanced glycation end product-specific receptor (gene AGER; UniProt Q15109, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 42.8 kDa |
| Observed MW | 45–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human RAGE. It differs from the mouse and rat sequences at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places advanced glycation end product-specific receptor in the cell membrane, cell projection, early endosome and nucleus. Tissue expression noted by UniProt (human): Endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Ying et al. (2016) Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway. SpringerPlus. 10.1186/s40064-016-1857-6
