Anti-RAGE/AGER Antibody Picoband®

Overview

Anti-RAGE/AGER Antibody Picoband® is an antibody for AGER detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: AGER (Tumor necrosis factor-inducible gene 6 protein); UniProt: Q15109
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 45-58 kDa, calculated 31203 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-RAGE/AGER Antibody Picoband® catalog # A03438-1.

Biological background

Biological context: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

Expression and localization notes: cellular localization: Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane., tissue context: Found in the synovial fluid of patients with rheumatoid arthritis. ..

Common research applications

• Western blotting (WB): Compare AGER levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of AGER in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify AGER-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The receptor for advanced glycation end products (RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. And RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane.
• Tissue details: Found in the synovial fluid of patients with rheumatoid arthritis. .
• Research category: Cancer,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Immunology,Innate Immunity,Invasion/Microenvironment,Macrophage/Inflammation,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.