| Field | Specification |
|---|---|
| Target | |
| Alternative names | Advanced glycosylation end product-specific receptor;Receptor for advanced glycosylation end products;AGER;RAGE; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RAGE |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOBF-1 is a rabbit monoclonal antibody that recognizes advanced glycation end product-specific receptor (gene AGER) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 42.8 kDa, and the supplier reports an observed band at about 48 kDa.
RAGE (AGER, receptor for advanced glycation end products) is a cell surface pattern recognition receptor of the immunoglobulin superfamily. It binds diverse stress-related ligands, including advanced glycation end products, S100 proteins, HMGB1 and amyloid-β oligomers, and triggers inflammatory signaling, so it is studied in diabetes complications, vascular disease, neurodegeneration and chronic inflammation.
| Target | Advanced glycation end product-specific receptor (gene AGER; UniProt Q15109, human) |
|---|---|
| Clone | AOBF-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.8 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): mouse lung.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RAGE.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places advanced glycation end product-specific receptor in the cell membrane, cell projection, early endosome and nucleus. Tissue expression noted by UniProt (human): Endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yueru Wang et al. (2026) Inflachromene attenuates monocrotaline-induced pulmonary arterial hypertension by suppressing the HMGB1–TLR4/RAGE–NF-κB signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2026.116712
Xiaoqin Zhang et al. (2024) Screening of active components of melastoma dodecandrum lour. against diabetic osteoporosis using cell membrane chromatography-mass spectrometry. Frontiers in Pharmacology. 10.3389/fphar.2024.1450154
Min Xiang et al. (2017) Effect of crocetin on vascular smooth muscle cells migration induced by advanced glycosylation end products. Microvascular Research. 10.1016/j.mvr.2017.02.004
Hongbin Wang et al. (2024) Puerarin alleviates the high glucose-induced oxidative stress via the RAGE/PKC/NOX4 axis in renal mesangial cells. Journal of Toxicological Sciences. 10.2131/jts.49.497
