| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ran-binding protein 9; RanBP9; BPM-L; BPM90; Ran-binding protein M; RanBPM; RanBP7 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RanBP9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25R64 is a rabbit monoclonal antibody that recognizes ran-binding protein 9 (gene RANBP9) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 77.8 kDa, and the supplier reports an observed band at about 91 kDa.
RanBP9 (RanBPM) is a scaffold protein found in the nucleus and cytoplasm. It is a core component of the CTLH E3 ubiquitin ligase complex and interacts with many receptors and signaling proteins, with roles reported in cell spreading, actin organization and neuronal signaling.
| Target | Ran-binding protein 9 (gene RANBP9; UniProt Q96S59, human) |
|---|---|
| Clone | 25R64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.8 kDa |
| Observed MW | 91 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ranBP9.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ran-binding protein 9 in the cytoplasm, nucleus and cell membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
