| Field | Specification |
|---|---|
| Target | |
| Alternative names | Regulatory-associated protein of mTOR;Raptor;p150 target of rapamycin (TOR)-scaffold protein;RPTOR;KIAA1303, RAPTOR; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Raptor |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EGB-18 is a rabbit monoclonal antibody that recognizes regulatory-associated protein of mTOR (gene RPTOR) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 149.0 kDa, and the supplier reports an observed band at about 149 kDa.
Raptor (RPTOR) is the defining scaffold subunit of mTOR complex 1 (mTORC1). It recruits substrates such as S6 kinase and 4E-BP1 through their TOS motifs and, with the Rag GTPases, helps bring mTORC1 to the lysosome in response to amino acids, so it is central to studies of nutrient sensing, growth and rapamycin sensitivity.
| Target | Regulatory-associated protein of mTOR (gene RPTOR; UniProt Q8N122, human) |
|---|---|
| Clone | EGB-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 149.0 kDa |
| Observed MW | 149 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
| Flow cytometry | 1:30 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human raptor.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places regulatory-associated protein of mTOR in the lysosome membrane, cytoplasm and cytoplasmic granule. Tissue expression noted by UniProt (human): Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
ZiQian Liu et al. (2026) YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR–HIF1α–LHDA Axis. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.71105
