| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone-binding protein RBBP4;Chromatin assembly factor 1 subunit C;CAF-1 subunit C;Chromatin assembly factor I p48 subunit;CAF-I 48 kDa subunit;CAF-I p48;Nucleosome-remodeling factor subunit RBAP48;Retinoblastoma-binding protein 4;RBBP-4;Retinoblastoma-binding protein p48;RBBP4;RBAP48; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RbAp48, identical to the related mouse sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets histone-binding protein RBBP4 in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 47.7 kDa, and the supplier reports an observed band at about 50–55 kDa.
RBBP4 (RbAp48) is a WD40-repeat histone-binding protein that helps chromatin regulators reach their histone targets. It is a shared subunit of several complexes, including CAF-1, NuRD, PRC2 and NURF, and so contributes to chromatin assembly after DNA replication, histone deacetylation and gene silencing. The protein is highly conserved.
| Target | Histone-binding protein RBBP4 (gene RBBP4; UniProt Q09028, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 47.7 kDa |
| Observed MW | 50–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human Jurkat cells, human U87 cells, human HepG2 cells, human K562 cells, human RT4 cells, human A549 cells, rat brain tissue, rat liver tissue, rat lung tissue, rat RH35 cells, mouse brain tissue, mouse liver tissue, mouse lung tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, mouse liver tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): mouse small intestine tissue, rat small intestine tissue, mouse liver tissue, human placenta tissue.
Samples with confirmed staining (ICC/IF): U2OS cells, A431 cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Synthetic peptide from the C-terminal region of human rbAp48. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places histone-binding protein RBBP4 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in neuroblastoma cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
