Anti-RBPMS Antibody Picoband®

Overview

Anti-RBPMS Antibody Picoband® is an antibody for RBPMS detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: RBPMS (RNA binding protein, mRNA processing factor); UniProt: Q93062
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 22 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-RBPMS Antibody catalog # A07130-2.

Biological background

Biological context: Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly (A) RNA.

Expression and localization notes: cellular localization: Nucleus. Cytoplasm. P-body., tissue context: Ubiquitously expressed, at various levels depending on the isoform and the tissue..

Common research applications

• Western blotting (WB): Compare RBPMS levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of RBPMS in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify RBPMS-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: RNA-binding protein with multiple splicing is a protein that in humans is encoded by the RBPMS gene. It is mapped to 8p12. This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. The RNA recognition motif is between 80-100 amino acids in length and family members contain one to four copies of the motif. The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. The encoded protein has a single, putative RNA recognition motif in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus. Cytoplasm. P-body.
• Tissue details: Ubiquitously expressed, at various levels depending on the isoform and the tissue.
• Research category: Cancer,DNA/RNA,Epigenetics and Nuclear Signaling,Ribosome,Translation,Tumor Biomarkers

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.