Anti-REA/PHB2 Antibody Picoband® (monoclonal, 2B5)

Overview

Anti-REA/PHB2 Antibody Picoband® (monoclonal, 2B5) is an antibody for PHB2 detection raised in Mouse (Monoclonal, clone Clone: 2B5, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: PHB2 (interleukin 32); UniProt: Q99623
• Antibody format: Mouse, Monoclonal, clone Clone: 2B5, Mouse IgG2b
• Molecular weight: 32 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-REA/PHB2 Antibody Picoband® (monoclonal, 2B5) catalog # M03315.

Biological background

Biological context: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Expression and localization notes: cellular localization: Secreted, tissue context: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle..

Common research applications

• Western blotting (WB): Compare PHB2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of PHB2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify PHB2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Secreted
• Tissue details: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
• Research category: ER Proteins,Immunology,Protein Trafficking,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.