| Field | Specification |
|---|---|
| Target | |
| Alternative names | Reversion-inducing cysteine-rich protein with Kazal motifs; hRECK; Suppressor of tumorigenicity 15 protein; RECK; ST15 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human RECK, which shares 88.6% and 79.5% amino acid (aa) sequence identity with mouse and rat RECK, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets reversion-inducing cysteine-rich protein with Kazal motifs (gene RECK) in human samples and is validated for Western blot.
RECK is a GPI-anchored membrane glycoprotein first identified as an inhibitor of matrix metalloproteinases. Together with GPR124 (ADGRA2) it acts as a specific co-receptor for Wnt7a and Wnt7b, enabling Wnt signaling in brain endothelial cells that is needed for CNS angiogenesis and blood–brain barrier formation.
| Target | Reversion-inducing cysteine-rich protein with Kazal motifs (gene RECK; UniProt O95980, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Immunogen
Synthetic peptide from the middle region of human RECK. Sequence identity with the mouse and rat orthologs: 88.6% and 79.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places reversion-inducing cysteine-rich protein with Kazal motifs in the cell membrane. Tissue expression noted by UniProt (human): Expressed in various tissues and untransformed cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiawei Zhang et al. (2021) Delivery of microRNA-21-sponge and pre-microRNA-122 by MS2 virus-like particles to therapeutically target hepatocellular carcinoma cells. Experimental Biology and Medicine. 10.1177/15353702211035689
Qi-xiang Liang et al. (2014) RECK overexpression reduces invasive ability in ameloblastoma cells. Journal of Oral Pathology & Medicine. 10.1111/jop.12179
