| Field | Specification |
|---|---|
| Target | |
| Alternative names | Renin; Angiotensinogenase; REN |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Renin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24R51 is a rabbit monoclonal antibody that recognizes renin in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 45.1 kDa, and the supplier reports an observed band at about 47 kDa.
Renin (REN) is an aspartic protease made and released mainly by juxtaglomerular cells of the kidney. It cuts angiotensinogen to form angiotensin I, the first and rate-limiting step of the renin–angiotensin system that raises blood pressure and promotes sodium retention, so renin levels are central to studies of blood pressure regulation and kidney physiology.
| Target | Renin (gene REN; UniProt P00797, human) |
|---|---|
| Clone | 24R51 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.1 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human renin.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes renin as secreted and also located in the membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
