| Field | Specification |
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| Alternative Names | Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
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| Target |
Overview
Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody is an antibody targeting Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2 (also reported as Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2).
- Immunogen/epitope region: Extracellular, N-terminus..
- Homology note: Rat, human - identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Specificity statement (as provided): This antibody will recognize both the membrane form of the Renin Receptor and the soluble cleaved form (sPRR)..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
The (pro) Renin receptor, (p)RR, encoded by ATP6AP2 gene in humans, is a single pass transmembrane protein that binds both Renin and its inactive precursor pro-renin. ATP6AP2 is highly conserved among species, and (Pro) Renin Receptor orthologues are expressed in species from mammals to C. elegans and Drospohila melanogaster1.Binding of Pro-renin to (p)RR receptor facilitates its truncation and generation of Renin.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-AR014.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-AR014; Negative control: BLP-AR014.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
