| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proto-oncogene tyrosine-protein kinase receptor Ret;2.7.10.1;Cadherin family member 12;Proto-oncogene c-Ret;Soluble RET kinase fragment;Extracellular cell-membrane anchored RET cadherin 120 kDa fragment;RET;CDHF12, CDHR16, PTC, RET51; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Ret |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FIF-18 is a rabbit monoclonal antibody that recognizes proto-oncogene tyrosine-protein kinase receptor Ret in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 124.3 kDa; the supplier reports observed bands at 150, 175 kDa.
RET is a receptor tyrosine kinase activated by GDNF-family ligands (GDNF, neurturin, artemin and persephin), which bind GFRα co-receptors and then engage RET. RET signaling is essential for kidney development and the enteric nervous system. Loss-of-function mutations cause Hirschsprung disease, while activating mutations and fusions drive multiple endocrine neoplasia type 2 and some thyroid and lung cancers.
| Target | Proto-oncogene tyrosine-protein kinase receptor Ret (gene RET; UniProt P07949, human) |
|---|---|
| Clone | FIF-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 124.3 kDa |
| Observed MW | 150, 175 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ret.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places proto-oncogene tyrosine-protein kinase receptor Ret in the cell membrane and endosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
